My MakerBot Printer Will Not Connect to MakerBot Desktop via USB Cable
Let's try reseating the USB cable on the printer end, and on the computer end. Does the software recognize the Printer now?
How about if you try different USB ports on the computer? Does the Printer continue to not connect?
If you have another A-to-B USB Cable give that a try.
If you are using a USB splitter, try connecting one-to-one from printer to computer.
Note: The Replicator+ and Replicator Mini+ cannot connect to MakerBot Desktop over USB. Please use MakerBot Print to Interface with those printers.